Kidney anatomy, histology and histometric traits associated to renosomatic index in Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae)

The Gymnotus inaequilabiatus is a Neotropical fish widely distributed in marginal areas of bays. The aim of this study was to describe the main histological and histopathological traits in the head and exocrine kidney. Here, histometric and structural density techniques were associated with renosomatic index (RSI). The kidney was processed for light microscopy. Lipofuscin and hemosiderin content were visually estimated in the melonomacrophages centers (MMCs). All the biometric body variables were correlated with RSI, especially the kidney weight and gross lesions count. The general architecture of head and exocrine kidney was similar to that described for other teleost species. MMCs were prevalent in both portions and correlated with RSI in the head and exocrine kidney. Granulomatous structures were often observed in both portions; however, they were associated only in the exocrine kidney with RSI. Of all the structures hystometrically estimated, only proximal tubular diameter and thickness, and distal tubular thickness were correlated to renosomatic index. The RSI is an useful biometric variable that represent some physiological and morphological characteristics of kidney in G. inaequilabiatus. These findings may be used in future studies to evaluate the effects of environmental stressors on the renal adaptative physiological process.(AU)

Gymnotus inaequilabiatus é um peixe neotropical amplamente distribuído em áreas marginais de baías. O objetivo deste estudo foi descrever características anatômicas, histológicas e histopatológicas no rim cefálico e exócrino. Análises histométricas e a densidade volumétrica estrutural foram associadas ao índice renossomático (IRS). Fragmentos de ambos tecidos foram processados para análise e microscopia de campo claro. O conteúdo de lipofuscina e hemossiderina foi estimado visualmente nos centros de melanomacrófagos (CMMs). Todas as variáveis corporais biométricas foram correlacionadas com o IRS, principalmente o peso renal e a contagem de lesões superficiais. A arquitetura geral do rim cefálico e exócrino foi semelhante à descrita para outras espécies de teleósteos. Os CMMs foram frequentes em ambas as porções e correlacionaram-se com o IRS. Estruturas granulomatosas foram frequentemente observadas em ambas porções; no entanto, foram associadas ao IRS apenas no rim exócrino. De todas as estruturas estimadas histometricamente, apenas o diâmetro e espessura dos túbulos proximais e distais correlacionaram-se ao IRS. Este índice mostrou-se uma variável útil associada a características fisiológicas e morfológicas do rim de G. inaequilabiatus. Esses achados podem ser empregados em futuros estudos a fim de avaliar efeitos ambientais bem como processos adaptativos fisiológicos.(AU)

Influence of rutin in renal tissue morphology in STZ-induced diabetes mice / 吉林大学学报(医学版)

Objective:To study the influence of rutin in the morphology of renal tissue of the diabetic mice induced by streptozotocin(STZ), and to clarify the effect of rutin on the kidney tissue damage.Methods:Twelve mice of the total 70 Kunming mice were used as normal group, the other were used to estabish type 1 diabetes mouse models by intraperitoneally injected with STZ (62.5 mg·kg-1), once daily for 5 d.The successfully established model mice were randomly divided into model group,low dose (50 mg·kg-1)of rutin group, high dose (100 mg·kg-1) of rutin group and irbesartan group (45 mg·kg-1).The mice in model group and normal group were given the carboxy methyl cellulose(CMC) and the other mice were given drugs by intragastric administration once daily for 8 weeks accordingly.The weight and blood glucose of the each mouse were determined.Full automatic biochemical analyzer was used to detect the levels of serum creatinine (Cre) and blood urine nitrogen (BUN) of the mice , and the kidney index was calculated.The morphology of renal tissue was observed by HE staining, Masson staining and electron microscope.Results:After injection of STZ,the model success rate was up to 98%.Compared with normal group, there was no significant difference in the weight of the mice in other groups before administration(P>0.05).After administration of rutin, the weights of the mice in model group, low dose of rutin group, high dose of rutin group and irbesartan group were significantly decreased (P<0.05).Compared with model group, the levels of blood glucose of the mice in low and high doses of rutin groups were significantly decreased(P<0.05);the levels of Cre and BUN were significantly reduced (P<0.05);the kidney index of the mice in high dose of rutin group was significantly decreased (P<0.05).Compared with normal group,the kidney tissue of the mice in model group was seriously damaged;glomerular was weaked, the kidney tissue fibrosis was serious, glomerular basement membrane was diffusely thickened and foot process was coalesced or overgrow.Compared with model group,the degree of injury of the mice in low and high doses of rutin groups were significantly improved, especially in high dose of rutin group.Conclusion:Rutin can improve the renal function of diabetic mice induced by STZ and reduce the degree of renal tissue damage in the diabetic mice

Morphology and dissection technique of the kidney of the grey snapper lutjanus griseus (teleostei: lutjanidae) / Morfología y técnica de disección del riñón del pargo gris lutjanus griseus (teleostei: lutjanidae)

In order to describe the morphology of the kidney and implement its dissection technique, specimens of the grey snapper Lutjanus griseus were analyzed. The morphology of the kidney fits with the structural pattern of the higher teleosts, consisting of a large fusion of the right kidney with the left, with the exception of the anterior renal lobes. Both anterior renal lobes are separated by the retractores dorsales muscles and by the anchorage of the gas bladder to the vertebrae. Some cranial and spinal nerves, as well as ribs, blood vessels, the Baudelot's ligament, the sinus venosus of the heart, the precaudal vertebrae, and the gas bladder mark the surface of the kidney. The archinephric ducts are connected with the urinary bladder, from which the common urinary and urogenital leaves.

Con el objeto de describir la morfología del riñón y de implementar su técnica de disección, se analizaron ejemplares del pargo gris Lutjanus griseus. La morfología del riñón corresponde al patrón estructural de los teleósteos superiores, consistiendo en una amplia fusión del riñón derecho con el izquierdo, con excepción de los lóbulos renales anteriores. Ambos lóbulos renales anteriores están separados por los músculos retractores dorsales y por el anclaje de la vejiga gaseosa a las vértebras. Algunas estructuras marcan la superficie del riñón, como algunos de los nervios craneales y espinales, las costillas, vasos sanguíneos, el ligamento de Baudelot, el seno venoso del corazón, las vértebras precaudales y la vejiga gaseosa. Los conductos arquinéfricos se conectan a la vesícula urinaria, desde la cual sale el conducto urinario común.